用保鮮膜從無殼雞蛋孵出小雞, 好神奇的胎盤。
youtube: https://www.youtube.com/watch?v=Vby8tCHkTLg
https://www.youtube.com/watch?v=Vby8tCHkTLg
論文: https://www.jstage.jst.go.jp/article/jpsa/51/3/51_0130043/_article
論文摘要 （Abstracts）:
The development of shell-less culture methods for bird embryos with high hatchability would be useful for the efficient generation of transgenic chickens, embryo manipulations, tissue engineering, and basic studies in regenerative medicine. To date, studies of culture methods for bird embryos include the whole embryo culture using narrow windowed eggshells, surrogate eggshells, and an artificial vessel using a gas-permeable membrane. However, there are no reports achieving high hatchability of >50% using completely artificial vessels. To establish a simple method for culturing chick embryos with high hatchability, we examined various culture conditions, including methods for calcium supplementation and oxygen aeration. In the embryo cultures where the embryos were transferred to the culture vessel after 55-56 h incubation, more than 90% of embryos survived until day 17 when a polymethylpentene film was used as a culture vessel with calcium lactate and distilled water supplementations. The aeration of pure oxygen to the surviving embryos from day 17 yielded a hatchability of 57.1% (8 out of 14). Thus, we successfully achieved a high hatchability with this method in chicken embryo culture using an artificial vessel.
簡單來說: 需要無菌狀態下、有鈣質提供、注意水份補充、要有事先培養箱培養55~56小時以上再取出、有純氧的提供、滿足這些條件後大約有57.1%的成功機率，小雞可以存活。
youtube 截圖:
